Maximum Score - 15 Points

Sample data table showing correct results of testing the specificity of the enzyme lactase.

Title: Testing for Enzyme Specificity

Spot	Substance Tested	Glucose present? yes	no
1	Glucose solution	X
2	Enzyme solution		X
3	Unknown A solution		X
4	Unknown B solution		X
5	Unknown C solution		X
6	Unknown A solution + Enzyme solution		X
7	Unknown B solution + Enzyme solution	X
8	Unknown C solution + Enzyme solution		X

Criteria:

Allow 1 point for each of the following;

• Appropriate title present.
• Substances tested are identified.
• Results of tests recorded and paired. Accuracy of results are not considered here.

Criteria:

2 points		Correct/generally states that well #2 acts as a control to show that the enzyme solution does not contain glucose. Answer should be written in complete sentences.
1 point		Correct statement, but not in complete sentences.
0 points		Incorrect responses, even if it is in complete sentences, or no response provided.

Criteria:

2 points		Correctly states that enzyme activity was indicated by the presence of glucose in spot 7. Answer should be written in complete sentences.
1 point		Correct statement, but not in complete sentences.
0 points		Incorrect responses, even if it is in complete sentences, or no response provided.

Criteria:

1 point		Correctly names lactose as the specific substrated/disaccharide on which lactase acts.
0 points		Incorrect responses, or no response provided.

Criteria:

1 point		Correctly identifies depression/spot/well 7 as containing the substrate.
0 points		Incorrect responses, or no response provided.

Criteria:

2 points		Correctly explains that in spot 7 chemical activity occurred as indicated by a positive test for glucose. Answer should be written in complete sentences.
1 point		Correct statement, but not in complete sentences.
0 points		Incorrect responses, or no response provided.

Criteria:

1 point		Correctly identifies this as a hydrolysis reaction.
0 points		Incorrect responses, or no response provided.

Criteria:

2 points		Correctly states that the lactose (disaccharide) is broken down into simpler molecules of glucose and galactose (monosaccharides) by the addition of a water molecule. Answer should be written in complete sentences.
1 point		Correct statement, but not in complete sentences.
0 points		Incorrect responses, or no response provided.